Bronson R A, Fusi F M, Fleit H B
Department of Obstetrics and Gynecology, State University of New York, Stony Brook 11794-8091.
J Reprod Immunol. 1992 Apr;21(3):293-307. doi: 10.1016/0165-0378(92)90032-y.
Oolemmal Fc receptors have previously been shown to play a role in the promotion of adhesion by antibody labeled human spermatozoa to zona-free hamster eggs. In this work, we demonstrated the presence of Fc gamma RI, Fc gamma RII and Fc gamma RIII on the oolemma of unfertilized human oocytes by means of monoclonal antibodies directed against these receptors, detected both by immunobead rosetting and indirect immunofluorescence. These receptors were also functionally active in that they were able to bind human aggregated IgG, human IgG-Fc, mouse IgG1 and IgG2a. While the presence of oolemmal IgG-Fc receptors might play a role in reproductive failure, by their promotion of polyspermic fertilization, in cases where antisperm antibodies bound to the spermatozoan surface, their role in the normal physiology of fertilization or in other events unrelated to sperm incorporation remains to be determined. In contrast, Fc gamma receptors were not present on human spermatozoa, irrespective of their functional state (fresh ejaculated, capacitated or acrosome reacted).
卵膜Fc受体先前已被证明在抗体标记的人类精子与去透明带仓鼠卵的黏附中发挥促进作用。在这项研究中，我们通过针对这些受体的单克隆抗体，借助免疫珠花环试验和间接免疫荧光检测，证实未受精的人类卵母细胞的卵膜上存在FcγRI、FcγRII和FcγRIII。这些受体在功能上也是活跃的，因为它们能够结合人聚合IgG、人IgG-Fc、小鼠IgG1和IgG2a。虽然卵膜IgG-Fc受体的存在可能通过促进多精受精在生殖失败中起作用，但在抗精子抗体结合到精子表面的情况下，它们在受精的正常生理过程或与精子纳入无关的其他事件中的作用仍有待确定。相比之下，无论人类精子的功能状态如何（新鲜射出、获能或顶体反应），其表面均不存在Fcγ受体。
