Fujita Y, Kobayashi M, Kobayashi K, Hoshino T
Central Laboratory for Electron Microscopy, Nagoya University School of Medicine, Japan.
J Electron Microsc (Tokyo). 1992 Apr;41(2):70-5.
Histochemical studies of epidermal Langerhans cells require well-preserved epidermal sheets. We studied the conditions for the preparation of the epidermal sheets from the skin of the ear, hind limb and trunk of nude mice. Two types of commercial dispase, and ethylenediaminetetraacetic acid (EDTA), were used at several concentrations and the effects were compared. Dermo-epidermal separation was evaluated by the preservation of ATPase activity of Langerhans cells in the epidermal sheet and by the ultrastructure of epidermal cells (Langerhans cells and keratinocytes) as well as epidermal-dermal junction. Good separation depended on the combination of the concentration of the reagent and site of the skin. The concentrations of both dispase and EDTA effective for separation of the epidermis in the nude mouse were lower than those in other mouse strains reported.
表皮朗格汉斯细胞的组织化学研究需要保存完好的表皮片。我们研究了从裸鼠耳部、后肢和躯干皮肤制备表皮片的条件。使用了两种不同的商业分散酶以及乙二胺四乙酸（EDTA），并设置了几个浓度水平，比较了它们的效果。通过表皮片中朗格汉斯细胞的ATP酶活性的保存情况、表皮细胞（朗格汉斯细胞和角质形成细胞）以及表皮 - 真皮连接的超微结构来评估真皮 - 表皮分离情况。良好的分离效果取决于试剂浓度和皮肤部位的组合。对裸鼠表皮分离有效的分散酶和EDTA的浓度均低于报道的其他小鼠品系。
