Baker K W, McKee-Protopapas S, Habowsky J E
Scan Electron Microsc. 1983(Pt 1):457-65.
Hairless mouse epidermis was separated from the underlying dermis using a 2 h incubation in 20 mM ethylenediaminetetraacetic acid (EDTA). The basal epidermis, thus exposed, was then examined using scanning electron (SEM), transmission electron (TEM), and light microscopy (LM). Sheets were also stained for: (i) Langerhans cell adenosine triphosphatase (ATPase), beta-glucuronidase, and la antigens; and, (ii) melanocyte 3,4-dihydroxyphenylalanine (DOPA)-oxidase. A regular distribution of protruding dendritic cells was observed superficial to the basal epidermis. These external dendritic cells were identified as Langerhans cells on the basis of subcellular morphology and distribution in the TEM. ATPase staining was Langerhans cell specific. The Langerhans cell population in hairless mouse epidermis was large, and evenly distributed in the interfollicular epidermis and the outer root sheath of degenerate hair follicles. The melanocyte population, in comparison, was negligibly small (4-5 cells per mm2).
使用20 mM乙二胺四乙酸（EDTA）孵育2小时，将无毛小鼠的表皮与下方的真皮分离。然后，使用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和光学显微镜（LM）对如此暴露的基底表皮进行检查。切片还进行了如下染色：（i）朗格汉斯细胞三磷酸腺苷酶（ATPase）、β-葡萄糖醛酸酶和la抗原；以及，（ii）黑素细胞3,4-二羟基苯丙氨酸（DOPA）氧化酶。在基底表皮表面观察到突出的树突状细胞呈规则分布。根据TEM中的亚细胞形态和分布，这些外部树突状细胞被鉴定为朗格汉斯细胞。ATPase染色具有朗格汉斯细胞特异性。无毛小鼠表皮中的朗格汉斯细胞群体很大，并且均匀分布在毛囊间表皮和退化毛囊的外根鞘中。相比之下，黑素细胞群体小到可以忽略不计（每平方毫米4 - 5个细胞）。
