Baker K W, Habowsky J E
J Invest Dermatol. 1983 Feb;80(2):104-7. doi: 10.1111/1523-1747.ep12531712.
Sheets of ethylenediaminetetraacetic acid (EDTA)-separated epidermis were examined using scanning electron, transmission electron, and light microscopy; sheets were also examined after staining for adenosine triphosphatase (ATPase) activity. Staining was improved by longer incubation with EDTA and by elimination of Trismal buffer as a tissue rinse. EDTA-separated epidermis showed better retention of ultrastructural integrity when washed with phosphate-buffered saline. The ATPase staining procedures described in this present study are ultrastructurally specific for the Langerhans cell.
使用扫描电子显微镜、透射电子显微镜和光学显微镜对乙二胺四乙酸（EDTA）分离的表皮片进行检查；在对三磷酸腺苷酶（ATPase）活性进行染色后也对表皮片进行了检查。通过延长与EDTA的孵育时间以及去除Trismal缓冲液作为组织冲洗液，染色效果得到了改善。当用磷酸盐缓冲盐水洗涤时，EDTA分离的表皮显示出更好的超微结构完整性保留。本研究中描述的ATPase染色程序在超微结构上对朗格汉斯细胞具有特异性。
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