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脉孢菌属中线粒体质粒的电泳图谱表明它们通过滚环机制进行复制。

Electrophoretic profiles of mitochondrial plasmids in Neurospora suggest they replicate by a rolling circle mechanism.

作者信息

Maleszka R

机构信息

Molecular and Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra.

出版信息

Biochem Biophys Res Commun. 1992 Aug 14;186(3):1669-73. doi: 10.1016/s0006-291x(05)81600-6.

DOI:10.1016/s0006-291x(05)81600-6
PMID:1387311
Abstract

Migratory behaviour of mitochondrial plasmids from Neurospora crassa Mauriceville-1c and N. intermediate LaBelle has been studied by pulsed field gel electrophoresis (PFGE). Electrophoretic profiles demonstrate that long, linear molecules of a heterogeneous size are the prevailing form of plasmid DNA in vivo. Circular forms represent less than 8-9% of plasmid DNA. Single stranded DNA regions are abundant and lead to electrophoretic inertia of a significant amount of plasmid DNA. These profiles indicate that both plasmids replicate by the recombination dependent rolling circle mechanism.

摘要

通过脉冲场凝胶电泳(PFGE)研究了粗糙脉孢菌Mauriceville-1c和中间脉孢菌LaBelle中线粒体质粒的迁移行为。电泳图谱表明,大小各异的长线性分子是体内质粒DNA的主要形式。环状形式占质粒DNA的比例不到8-9%。单链DNA区域丰富,导致大量质粒DNA出现电泳惰性。这些图谱表明这两种质粒均通过依赖重组的滚环机制进行复制。

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