BRUBAKER R R, SURGALLA M J
J Bacteriol. 1961 Dec;82(6):940-9. doi: 10.1128/jb.82.6.940-949.1961.
Brubaker, Robert R. (U. S. Army Biological Laboratories, Fort Detrick, Frederick, Md.) and Michael J. Surgalla. Pesticins. I. Pesticin-bacterium interrelationships, and environmental factors influencing activity. J. Bacteriol. 82: 940-949. 1961-A second bacteriocin-like substance produced by all tested strains of Pasteurella pestis and P. pseudotuberculosis is described. This activity, termed pesticin II, is active against the two avirulent P. pestis strains, A12 and Java. These strains do not produce pesticin I, which inhibits the growth of type I strains of P. pseudotuberculosis. Pesticin I was also found to be active against certain strains of Escherichia coli, strain A12, but not strain Java, and some P. pestis isolates which also produce pesticin I. A number of E. coli strains produce a substance which also inhibits the growth of strains A12 and Java; the activity of this substance is dependent upon the presence of high concentrations of Ca(++). The activity of both pesticins is inhibited under anaerobic conditions or in the presence of antiserum. The activity of pesticin I, but not pesticin II, is suppressed by Fe(+++), hemin, certain hemin-containing proteins, Mg(++), and inorganic phosphate. Suppression of pesticin I activity by Fe(+++) can be reversed by the addition of either Ca(++) or Sr(++) or by metal chelating agents. All tested strains of P. pestis and P. pseudotuberculosis produce a metabolite which suppresses the activity of pesticin I. The activity of this substance, termed pesticin I inhibitor, is enhanced by Fe(+++), and to a lesser extent by Mg(++) or by inorganic phosphate; its activity is suppressed by Mn(++) and by protamine sulfate.
布鲁贝克，罗伯特·R.（美国陆军生物实验室，马里兰州弗雷德里克市德特里克堡）和迈克尔·J. 苏尔加拉。鼠疫菌素。I. 鼠疫菌素与细菌的相互关系以及影响活性的环境因素。《细菌学杂志》82: 940 - 949。1961年——描述了由所有测试的鼠疫耶尔森菌和假结核耶尔森菌菌株产生的第二种类细菌素物质。这种活性物质被称为鼠疫菌素II，对两种无毒力的鼠疫耶尔森菌菌株A12和爪哇菌株有活性。这些菌株不产生抑制假结核耶尔森菌I型菌株生长的鼠疫菌素I。还发现鼠疫菌素I对某些大肠杆菌菌株（A12菌株，但不对爪哇菌株）以及一些也产生鼠疫菌素I的鼠疫耶尔森菌分离株有活性。许多大肠杆菌菌株产生一种也抑制A12和爪哇菌株生长的物质；这种物质的活性取决于高浓度Ca(++)的存在。两种鼠疫菌素的活性在厌氧条件下或存在抗血清时均被抑制。鼠疫菌素I的活性，但不是鼠疫菌素II的活性，被Fe(+++)、血红素、某些含血红素的蛋白质、Mg(++)和无机磷酸盐抑制。通过添加Ca(++)或Sr(++)或金属螯合剂可以逆转Fe(+++)对鼠疫菌素I活性的抑制。所有测试的鼠疫耶尔森菌和假结核耶尔森菌菌株都产生一种抑制鼠疫菌素I活性的代谢产物。这种物质被称为鼠疫菌素I抑制剂，其活性被Fe(+++)增强，在较小程度上被Mg(++)或无机磷酸盐增强；其活性被Mn(++)和硫酸鱼精蛋白抑制。
