Gramotina L I, Suchkov Iu G, Rozanova G N
Antibiotiki. 1982 Aug;27(8):588-92.
It was found that 96.2 per cent of the plague microbe cultures studied had a capacity to produce pesticin I on solid and liquid minimum synthetic nutrient media providing growth of the cultures. No pesticin III was detected in the spheroplasts of plague microbes, when they were grown on the synthetic nutrient medium, while on the complete nutrient medium the inhibition zones were observed which was indicative of pesticin III synthesis. The average value characterizing the titer of pesticin I produced by the rod-shaped forms of the plague microbe on the complete and synthetic nutrient media was 320. An increase in the titer of the spheroplast pesticin was shown, while the pesticin titer of the rod-shaped forms was low (1 : 20--1 : 320). As a result the average titer of the spheroplast pesticin was higher than that of pesticin of the rod-like forms. On the complete nutrient medium it was 1280 and on the synthetic medium it was 640.
研究发现,在所研究的鼠疫微生物培养物中,96.2% 在能使培养物生长的固体和液体最低合成营养培养基上具有产生鼠疫菌素I的能力。当鼠疫微生物原生质体在合成营养培养基上生长时,未检测到鼠疫菌素III,而在完全营养培养基上观察到抑菌圈,这表明有鼠疫菌素III的合成。鼠疫微生物杆状形态在完全和合成营养培养基上产生的鼠疫菌素I效价的平均值为320。结果显示原生质体鼠疫菌素效价有所增加,而杆状形态的鼠疫菌素效价较低(1:20 - 1:320)。因此,原生质体鼠疫菌素的平均效价高于杆状形态鼠疫菌素的平均效价。在完全营养培养基上为1280,在合成培养基上为640。