Ramieri G, Panzica G C, Viglietti-Panzica C, Modica R, Springall D R, Polak J M
Department of Stomatology, University of Torino, Italy.
Arch Oral Biol. 1992 Apr;37(4):263-9. doi: 10.1016/0003-9969(92)90048-d.
Merkel cells are non-keratinized cells present in many different epithelia, but whose origin and functional role are still controversial. They were here investigated by means of antisera to the neural and neuroendocrine markers protein gene product 9.5 (PGP 9.5), and neurone-specific enolase. The expression of both markers in Merkel cells of human gingival and palatal mucosa was confirmed. Merkel cell-neurite complexes and isolated non-innervated Merkel cells had a similar morphology when stained by either antiserum. Merkel-neurite complexes were clustered in relatively large numbers in the lingual gingiva, thus constituting structures closely similar to the 'touch domes' in the skin. Clusters of non-innervated cells showing the same immunohistochemical features as Merkel cells were also demonstrated. In other areas of the oral mucosa, the innervated and non-innervated elements were only occasionally seen but there were many encapsulated Meissner-like receptors. When comparing the two different antisera, anti-PGP 9.5 appeared to provide a more consistent labelling of small fibres inside the epithelium and of bulb-like terminals on Merkel cells.
默克尔细胞是存在于许多不同上皮组织中的非角质化细胞，但其起源和功能作用仍存在争议。本文通过针对神经和神经内分泌标志物蛋白基因产物9.5（PGP 9.5）以及神经元特异性烯醇化酶的抗血清对其进行了研究。证实了这两种标志物在人牙龈和腭黏膜的默克尔细胞中的表达。当用任何一种抗血清染色时，默克尔细胞 - 神经突复合体和孤立的无神经支配的默克尔细胞具有相似的形态。默克尔 - 神经突复合体在舌侧牙龈中大量聚集，从而构成与皮肤中的“触觉小体”非常相似的结构。还证实了存在具有与默克尔细胞相同免疫组织化学特征的无神经支配细胞簇。在口腔黏膜的其他区域，仅偶尔可见有神经支配和无神经支配的成分，但有许多被囊的类梅斯纳受体。比较两种不同的抗血清时，抗PGP 9.5似乎能更一致地标记上皮内的小纤维以及默克尔细胞上的球状终末。
