用间接免疫荧光法检测大鼠牙髓和牙周组织中降钙素基因相关肽的表达。
Expression of calcitonin gene-related peptide in rat pulp and periodontal tissues by indirect immunofluorescence method.
作者信息
Sun Yingming, Tao Rui, Zhang Mengjie, Cao Xue, Wang Hong, Xue Lufeng, Wu Meng
机构信息
Department of Stomatology, The 101 Military Hospital , Wuxi, China .
出版信息
Monoclon Antib Immunodiagn Immunother. 2013 Dec;32(6):404-8. doi: 10.1089/mab.2013.0044.
Abstract
The aim of this study was to investigate the expression of nerve fibers immunoreactive to calcitonin gene-related peptide (CGRP) in pulp and periodontal tissues of rats. Male Sprague-Dawley rats, aged 6 weeks, were sacrificed, and the jaws were excised, demineralized, and processed for indirect immunofluorescence staining. A considerably higher density of nerve fibers immunoreactive to CGRP was found in the dental pulp and gingiva than in periodontal ligament. The majority of pulpal CGRP immunopositive fibers that were located followed blood vessels parallel to the long axis of the root. A subodontoblastic network of fibers IR to CGRP was found in the coronal pulp in rat molars. In the periodontium, CGRP immunopositive fibers were mainly located in the periapical area and close to the alveolar bone. Gingiva was also well supplied with CGRP-IR nerves.
摘要
本研究旨在调查大鼠牙髓和牙周组织中对降钙素基因相关肽（CGRP）免疫反应的神经纤维的表达。处死6周龄雄性Sprague-Dawley大鼠，切除颌骨，脱矿，并进行间接免疫荧光染色处理。发现牙髓和牙龈中对CGRP免疫反应的神经纤维密度明显高于牙周韧带。大多数位于牙髓的CGRP免疫阳性纤维沿与牙根长轴平行的血管分布。在大鼠磨牙的冠髓中发现了一个成牙本质细胞下CGRP免疫反应纤维网络。在牙周组织中，CGRP免疫阳性纤维主要位于根尖周区域并靠近牙槽骨。牙龈也有丰富的CGRP免疫反应神经供应。
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