Muller S, Richalet P, Laurent-Crawford A, Barakat S, Rivière Y, Porrot F, Chamaret S, Briand J P, Montagnier L, Hovanessian A
Immunochemistry Laboratory (UPR 9002-CNRS), Institute of Molecular and Cellular Biology, Strasbourg, France.
AIDS. 1992 Sep;6(9):933-42. doi: 10.1097/00002030-199209000-00004.
To analyse serological aspects of systemic autoimmunity in HIV-1-seropositive patients and in individuals at risk for AIDS.
The reactivity of antibodies in the serum of 100 HIV-1-seropositive patients was investigated by enzyme-linked immunosorbent assay (ELISA) using a series of antigens known to be recognized by antibodies from patients with multisystemic autoimmune diseases, such as systemic lupus erythematosus, mixed-connective tissue disease and Sjögren's syndrome.
High levels of immunoglobulin G (IgG) antibodies reacting with double-stranded DNA (dsDNA), synthetic peptides of ubiquitinated histone H2A, Sm-D antigen, U1-A RNP antigen and 60 kD SSA/Ro antigen were found in 44-95% of HIV-infected patients. Among histone antibodies, the most frequent reactions were towards the carboxy-terminal region of histone H1 and to histone H2B and its amino-terminal domain 1-25. Eight HIV-1-seropositive patients at different stages of disease according to the Centers for Disease Control classification were also studied. In most cases, no obvious fluctuations were observed over several years. Antibodies were found early, and their specificity and apparent level of activity remained relatively constant. There was no evidence of such an autoimmune response in the serum of high-risk homosexual seronegative men.
Although the aetiology of AIDS is known, in general the aetiology of multisystemic autoimmune diseases remains to be determined, and the sequence of events taking place remains obscure in both cases. It is possible that the large spectrum of antibodies found in HIV-infected patients reflects a specific stimulation of B-cells by nuclear antigens released by apoptosis during an early stage of disease.
分析HIV-1血清阳性患者及艾滋病高危个体系统性自身免疫的血清学特征。
采用酶联免疫吸附测定（ELISA），使用一系列已知可被多系统自身免疫性疾病（如系统性红斑狼疮、混合性结缔组织病和干燥综合征）患者抗体识别的抗原，研究100例HIV-1血清阳性患者血清中抗体的反应性。
在44%-95%的HIV感染患者中发现了高水平的免疫球蛋白G（IgG）抗体，这些抗体与双链DNA（dsDNA）、泛素化组蛋白H2A的合成肽、Sm-D抗原、U1-A核糖核蛋白抗原和60kD SSA/Ro抗原发生反应。在组蛋白抗体中，最常见的反应是针对组蛋白H1的羧基末端区域以及组蛋白H2B及其氨基末端结构域1-25。还研究了根据疾病控制中心分类处于不同疾病阶段的8例HIV-1血清阳性患者。在大多数情况下，数年内未观察到明显波动。抗体发现较早，其特异性和明显的活性水平保持相对恒定。在高危同性恋血清阴性男性的血清中未发现这种自身免疫反应的证据。
尽管艾滋病的病因已知，但一般来说，多系统自身免疫性疾病的病因仍有待确定，两种情况下发生的事件顺序仍不清楚。HIV感染患者中发现的大量抗体可能反映了疾病早期凋亡释放的核抗原对B细胞的特异性刺激。
