Leypoldt J K, Gilson J F, Blindauer K M, Cheung A K
Medical Service, VA Medical Center, Salt Lake City, UT 84148.
Blood Purif. 1992;10(1):53-60. doi: 10.1159/000170074.
Adsorption to hemodialysis membranes was studied by determining the binding kinetics of model macromolecules, polydisperse DEAE dextran (molecular radii 10-70 A), to an acrylonitrile-methallyl sulfonate copolymer membrane. Hemodialyzers were studied in a postdilution hemofiltration circuit where both blood path output and ultrafiltrate streams were returned to the reservoir. Changes in the reservoir concentration of and sieving coefficients for DEAE dextran were monitored over 24 h. Decreases (or increases) in reservoir concentration were assumed to result from adsorption to (or desorption from) the membrane. Small macromolecules adsorbed rapidly to the membrane but later desorbed. This rapid adsorption resulted in low initial sieving coefficients. Large macromolecules adsorbed slowly over the entire 24-hour study period. Additional experiments suggested that desorption of small macromolecules was due to displacement by large macromolecules. Adsorption and desorption of macromolecules to hemodialysis membranes are dynamic processes and depend on molecular size.
通过测定模型大分子——多分散性二乙氨基乙基葡聚糖（分子半径为10 - 70埃）与丙烯腈 - 甲基烯丙基磺酸盐共聚物膜的结合动力学，研究了其在血液透析膜上的吸附情况。在后置稀释血液滤过回路中对血液透析器进行研究，其中血液通路输出液和超滤液流都返回储液器。在24小时内监测储液器中二乙氨基乙基葡聚糖的浓度变化和筛分系数。储液器浓度的降低（或升高）被认为是由于膜吸附（或解吸）所致。小分子迅速吸附到膜上，但随后解吸。这种快速吸附导致初始筛分系数较低。大分子在整个24小时研究期间吸附缓慢。额外的实验表明，小分子的解吸是由于大分子的置换作用。大分子在血液透析膜上的吸附和解吸是动态过程，并且取决于分子大小。
