Tam J E, Knight S T, Davis C H, Wyrick P B
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Biotechniques. 1992 Sep;13(3):374-8.
The use of microcarrier cell culture as a method for the in vitro propagation of the obligate intracellular bacterial parasite, Chlamydia trachomatis, is described. The microcarrier beads proved to be a more cost-effective means to propagate C. trachomatis than traditional tissue culture flasks or roller bottles without sacrificing yields or infectivity. In addition, microcarrier cell culture was found to be a much simpler technique to study the intracellular development of these bacteria.
本文描述了使用微载体细胞培养作为专性细胞内细菌寄生虫沙眼衣原体体外繁殖方法的情况。事实证明，与传统的组织培养瓶或滚瓶相比，微载体珠是一种更具成本效益的繁殖沙眼衣原体的方法，且不会牺牲产量或感染力。此外，发现微载体细胞培养是研究这些细菌细胞内发育的一种更为简单的技术。
