Wyrick P B, Gerbig D G, Knight S T, Raulston J E
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599-7290, USA.
Microb Pathog. 1996 Jan;20(1):31-40. doi: 10.1006/mpat.1996.0003.
Chlamydia trachomatis serovar E is a major cause of bacterially-acquired sexually transmitted infections. Stock cultures of these obligate intracellular bacteria are often propogated in McCoy cells. We recently reported that greater infectious titers of chlamydiae could be obtained if the McCoy cells were cultured on collagen-coated microcarrier beads versus plastic flasks, although the reason for the difference in efficiency was not clear. This study analyzed the development of C. trachomatis grown in McCoy cells by the two methods. Transmission electron microscopy analysis revealed an accelerated chlamydial development, with maturation of reticulate bodies into elementary bodies sooner in McCoy cells grown on the porous substratum. Comparison of particle counts versus infectivity titers indicated the production of fewer numbers of elementary bodies but which were highly infectious sooner from the infected McCoy cell-microcarrier bead cultures than from duplicate infected McCoy cell cultures grown in plastic tissue culture flasks.
沙眼衣原体血清型E是细菌性性传播感染的主要病因。这些专性胞内细菌的储备培养物通常在McCoy细胞中繁殖。我们最近报道，如果将McCoy细胞培养在胶原包被的微载体珠上而不是塑料培养瓶中，可以获得更高的衣原体感染滴度，尽管效率差异的原因尚不清楚。本研究分析了通过这两种方法在McCoy细胞中生长的沙眼衣原体的发育情况。透射电子显微镜分析显示衣原体发育加速，在多孔基质上生长的McCoy细胞中，网状体更快成熟为原体。颗粒计数与感染滴度的比较表明，原体数量较少，但与在塑料组织培养瓶中生长的重复感染McCoy细胞培养物相比，感染的McCoy细胞-微载体珠培养物中产生的原体更早具有高感染性。
