Saitoh K, Chiba T, Nakamura K
Department of Pathology, National Medical Center, Tokyo, Japan.
Eur J Cancer. 1992;28A(10):1642-6. doi: 10.1016/0959-8049(92)90059-b.
Cell proliferation kinetics of human gastric carcinoma were studied immunohistochemically using a monoclonal antibody against DNA polymerase-alpha (Pol-alpha). The distribution patterns and percentages of proliferative cells were examined in cases with various histological types of gastric carcinoma and compared with those of normal epithelium of the gastric foveolae. Pol-alpha-positive epithelial cells were localised at the isthmus of the normal foveola, while Pol-alpha-positive cancer cells were distributed irregularly in the cancer nests. The percentage of Pol-alpha-positive cells (%PPC) was significantly higher in the carcinoma [mean (S.D.) 41.6 (12.9)%] than in the normal foveola [24.8 (6.4)%] (P < 0.01). Also, the intestinal-type carcinoma showed a relatively higher %PPC [44.9 (12.0)%] than the diffuse type [36.2 (15.1)%] (P<0.05), and the %PPC of signet ring cell carcinoma was extremely low [7.3 (2.2)%] (P< 0.01). Pol-alpha-positive cancer cells were observed most abundantly in the lamina propria of the mucosa. They decreased in number with the depth of cancer infiltration down to the subserosa.
利用抗DNA聚合酶α（Pol-α）单克隆抗体，通过免疫组织化学方法研究了人胃癌的细胞增殖动力学。在不同组织学类型的胃癌病例中检查增殖细胞的分布模式和百分比，并与胃小凹正常上皮的分布模式和百分比进行比较。Pol-α阳性上皮细胞位于正常小凹的峡部，而Pol-α阳性癌细胞在癌巢中分布不规则。癌组织中Pol-α阳性细胞百分比（%PPC）[平均（标准差）41.6（12.9）%]显著高于正常小凹[24.8（6.4）%]（P<0.01）。此外，肠型癌的%PPC[44.9（12.0）%]相对高于弥漫型[36.2（15.1）%]（P<0.05），印戒细胞癌的%PPC极低[7.3（2.2）%]（P<0.01）。在黏膜固有层中观察到Pol-α阳性癌细胞数量最多。随着癌浸润深度至浆膜下，其数量减少。
