Isetani K, Hosokawa Y, Wakabayashi N, Tokita K, Mitsufuji S, Maruyama K, Kodama T, Kashima K, Tsuchihashi Y
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1993 Feb;90(2):91-6.
To evaluate correctly the proliferative activity of tumor cells, it is necessary to clarify not only S-phase fraction but also the growth fraction of tumor tissues. We used combined BrdU and DNA polymerase alpha (pol-alpha) immunohistochemistry to gastric biopsy specimens, and analyzed the proliferation of the neoplastic lesions of various degrees of malignancy. The results were as follows: The distribution of pol-alpha positive cells were almost the same as that of BrdU positive cells, but the percentage of pol-alpha positive cells was higher than that of BrdU positive cells irrespective of the mucosal specimens. In the adenomas, both BrdU and pol-alpha positive cells distributed generally superficially in the mucosal layer. In the well differentiated adenocarcinomas, both BrdU and pol-alpha positive cells distributed diffusely in the deeper layer of the mucosa. The ratio of the number of BrdU positive cells to that of pol-alpha positive cells, which means the S-phase fraction in the growth fraction, was higher in the tumor and that higher in the well differentiated adenocarcinomas than that of the adenomas. In conclusion, the combined BrdU and pol-alpha immunohistochemistry in gastric biopsy specimens are useful to evaluate the degree of malignancy.
为了正确评估肿瘤细胞的增殖活性，不仅有必要明确S期分数，还需明确肿瘤组织的生长分数。我们将BrdU和DNA聚合酶α（pol-α）免疫组织化学联合应用于胃活检标本，并分析了不同恶性程度肿瘤病变的增殖情况。结果如下：pol-α阳性细胞的分布与BrdU阳性细胞的分布几乎相同，但无论黏膜标本如何，pol-α阳性细胞的百分比均高于BrdU阳性细胞。在腺瘤中，BrdU和pol-α阳性细胞通常分布在黏膜层的浅表部位。在高分化腺癌中，BrdU和pol-α阳性细胞均弥漫分布于黏膜的深层。BrdU阳性细胞数与pol-α阳性细胞数的比值，即生长分数中的S期分数，在肿瘤中较高，且在高分化腺癌中比腺瘤更高。总之，BrdU和pol-α免疫组织化学联合应用于胃活检标本有助于评估恶性程度。
