Shah H N, Gharbia S E
Department of Oral Biology, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Int J Syst Bacteriol. 1992 Oct;42(4):542-6. doi: 10.1099/00207713-42-4-542.
A total of 31 strains of Prevotella intermedia were subjected to DNA-DNA hybridization and were characterized by performing physiological tests and by performing a multilocus enzyme analysis, using malate dehydrogenase and glutamate dehydrogenase. All of the strains assigned to P. intermedia fermented glucose and sucrose, hydrolyzed starch but not esculin, and produced indole, acetic, isobutyric, isovaleric, and succinic acids as metabolic end products. The results of DNA reassociation experiments performed with the reference probe permitted separation of the strains into two well-defined homology groups. In addition, strains with DNAs that hybridized with DNA from strain ATCC 25611T (T = type strain) had high levels of peptidase activity and cleaved lipid substrates (4-methylumbelliferyl laurate and 4-methylumbellifelyl elaidate). Multilocus enzyme electrophoresis revealed two electromorphic profiles, one characteristic of strain ATCC 25611T and the other characteristic of strain ATCC 33563T. We propose that a new species, Prevotella nigrescens, should be created for the genetically distinct group of strains that hybridized with strain ATCC 33563T. Strain ATCC 33563 is designated the type strain of P. nigrescens.
总共31株中间普氏菌进行了DNA - DNA杂交，并通过进行生理测试以及使用苹果酸脱氢酶和谷氨酸脱氢酶进行多位点酶分析来进行特征鉴定。所有归为中间普氏菌的菌株都能发酵葡萄糖和蔗糖，水解淀粉但不水解七叶苷，并产生吲哚、乙酸、异丁酸、异戊酸和琥珀酸作为代谢终产物。用参考探针进行的DNA重结合实验结果使这些菌株可分为两个明确的同源组。此外，与ATCC 25611T菌株（T = 模式菌株）的DNA杂交的菌株具有高水平的肽酶活性，并能裂解脂质底物（4 - 甲基伞形酮月桂酸酯和4 - 甲基伞形酮反油酸酯）。多位点酶电泳揭示了两种电泳图谱，一种是ATCC 25611T菌株的特征图谱，另一种是ATCC 33563T菌株的特征图谱。我们建议为与ATCC 33563T菌株杂交的基因不同的菌株组创建一个新物种，即变黑普氏菌。菌株ATCC 33563被指定为变黑普氏菌的模式菌株。
