Sakamoto Mitsuo, Kumada Hidefumi, Hamada Nobushiro, Takahashi Yusuke, Okamoto Masaaki, Bakir Mohammad Abdul, Benno Yoshimi
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):319-22. doi: 10.1099/ijs.0.002626-0.
Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus Prevotella. These strains were most closely related to Prevotella intermedia ATCC 25611(T), with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were Prevotella pallens and Prevotella nigrescens (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of P. intermedia JCM 12248(T) and P. nigrescens JCM 12250(T). The isolates could be differentiated from P. pallens JCM 11140(T) on the basis of mannose fermentation and alpha-fucosidase activity. The isolates could not be distinguished from P. intermedia or P. nigrescens using conventional biochemical tests. DNA-DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to P. pallens JCM 11140(T), P. intermedia JCM 12248(T) and P. nigrescens JCM 12250(T). On the basis of these data, strains 04013, 04021, 04043, 04052(T), 0406, 04113, 04111 and 04161 represent a novel Prevotella species, for which the name Prevotella falsenii sp. nov. is proposed. The type strain is 04052(T) (=JCM 15124(T) =CCUG 56137(T)).
从猴口腔中分离出的8株厌氧、产色素、无芽孢、革兰氏阴性、杆状菌株，进行了表型和化学分类学特征分析，并通过16S rRNA基因序列分析确定了它们的系统发育位置。16S rRNA基因序列分析表明，这些分离株代表普雷沃菌属的一个单一物种。这些菌株与中间普雷沃菌ATCC 25611(T)关系最为密切，16S rRNA基因序列相似性为95.0%。其次关系最密切的物种是苍白普雷沃菌和变黑普雷沃菌（与各自模式菌株的相似性分别为92.7%和92.1%）。分离株的表型和生化特征与中间普雷沃菌JCM 12248(T)和变黑普雷沃菌JCM 12250(T)相同。基于甘露糖发酵和α-岩藻糖苷酶活性，分离株可与苍白普雷沃菌JCM 11140(T)区分开来。使用传统生化试验无法区分分离株与中间普雷沃菌或变黑普雷沃菌。DNA-DNA杂交实验揭示了这8株菌株相对于苍白普雷沃菌JCM 11140(T)、中间普雷沃菌JCM 12248(T)和变黑普雷沃菌JCM 12250(T)的基因组独特性。基于这些数据，菌株04013、04021、04043、04052(T)、0406、04113、04111和04161代表一个新的普雷沃菌种，为此提出名称为虚假普雷沃菌（Prevotella falsenii）新种。模式菌株为04052(T)（=JCM 15124(T)=CCUG 56137(T)）。
