Rinaudo M, Le Dung P, Gey C, Milas M
Centre de Recherches sur les Macromolécules Végétales, University Joseph Fourier of Grenoble, France.
Int J Biol Macromol. 1992 Jun;14(3):122-8. doi: 10.1016/s0141-8130(05)80001-7.
The use of the n.m.r. method in the investigation of chitosan carboxymethylation was evaluated. It seems to be the most effective technique to determine concurrently the degree and the position of substitution of the carboxymethylated chitosan derivatives. The 13C-n.m.r., by the DEPT method, 1H-1H and 1H-13C-n.m.r. correlations give much valuable information from the chemical shifts of the complex carboxymethylchitosan spectra. The relative reactivity of the functional groups of chitosan towards carboxymethylation was also determined assuming a higher reactivity of the C-6 position.
评估了核磁共振（n.m.r.）方法在壳聚糖羧甲基化研究中的应用。它似乎是同时测定羧甲基化壳聚糖衍生物取代度和取代位置的最有效技术。通过DEPT方法的¹³C-n.m.r.、¹H-¹H和¹H-¹³C-n.m.r.相关性，从复杂的羧甲基壳聚糖光谱的化学位移中获得了许多有价值的信息。假设C-6位置具有更高的反应活性，还测定了壳聚糖官能团对羧甲基化的相对反应活性。
