Shigemasa Y, Matsuura H, Sashiwa H, Saimoto H
Department of Materials Science, Faculty of Engineering, Tottori University, Japan.
Int J Biol Macromol. 1996 Apr;18(3):237-42. doi: 10.1016/0141-8130(95)01079-3.
Various acetylated chitosan derivatives and mixtures of chitin and chitosan, covering the range of the degree of deacetylation (DDA) from 0-100% were analyzed by 1H-NMR spectroscopy and infrared spectroscopy. The use of the 1070 cm-1 or 1030 cm-1 absorption band as an internal standard in the determination of DDA from the absorbance of the amide I bands at 1655 cm-1 and 1630 cm-1 or the amide II band at 1560 cm-1 was studied. There is a good correlation between the results from IR spectroscopy and those from 1H-NMR spectroscopy.
通过1H-核磁共振光谱法和红外光谱法分析了脱乙酰度（DDA）范围为0-100%的各种乙酰化壳聚糖衍生物以及几丁质与壳聚糖的混合物。研究了在根据1655 cm-1和1630 cm-1处酰胺I带或1560 cm-1处酰胺II带的吸光度测定DDA时，使用1070 cm-1或1030 cm-1吸收带作为内标的情况。红外光谱法的结果与1H-核磁共振光谱法的结果之间存在良好的相关性。
