Tani T, Chang T M, Kodama M, Tsuchiya M
First Department of Surgery, Shiga University of Medical Science, Nippon.
Biomater Artif Cells Immobilization Biotechnol. 1992;20(2-4):457-62. doi: 10.3109/10731199209119669.
Endotoxin contamination in modified hemoglobin can result in side effects. Accurate measurement and effective elimination of endotoxin are important in producing safe hemoglobin preparation. A new turbidometric endotoxin assay (Toxinometer) was studied, in which absorbance of wavelength was applied at 660 nm. This method is not affected by the presence of hemoglobin in solution. This way, toxinometer can accurately measure endotoxin concentration in hemoglobin solution. For the elimination of endotoxin, polymyxin-B immobilized fiber (PMX-F) was studied in-vitro and compared with commercial materials. The PMX-F was found to be a convenient and less expensive approach.
修饰血红蛋白中的内毒素污染可导致副作用。准确测量和有效去除内毒素对于制备安全的血红蛋白制剂至关重要。研究了一种新的比浊法内毒素测定法（毒素测定仪），该方法在660nm波长下测定吸光度。该方法不受溶液中血红蛋白的影响。通过这种方式，毒素测定仪可以准确测量血红蛋白溶液中的内毒素浓度。为了去除内毒素，对固定有多粘菌素B的纤维（PMX-F）进行了体外研究，并与市售材料进行了比较。结果发现PMX-F是一种方便且成本较低的方法。
