Kamiya M, Ueda G, Miyakawa M, Arai Y, Shirai T
Department of Anatomy, Juntendo University School of Medicine, Tokyo, Japan.
Brain Behav Immun. 1992 Sep;6(3):300-6. doi: 10.1016/0889-1591(92)90051-o.
Autoimmune-prone New Zealand Black mice produce a large amount of autoantibodies cytotoxic for thymocytes (natural thymocytotoxic autoantibodies, NTA). A monoclonal NTA (NTA260) has been found to react with brain tissues as well as the cell surface of thymocytes. We investigated the expression of NTA 260 antigen in the primary culture of fetal brain cells. NTA260 labeled strongly the cytoplasm of nerve cells after fixation, but failed to stain the living cells. Western blot analysis revealed that NTA260 recognized predominantly a band at approximately 53 kDa in brain and thymic extracts. These findings indicate that neuronal NTA260 antigen, which has a molecular mass similar to that of thymocytes, is likely an intracellular component.
易患自身免疫病的新西兰黑鼠会产生大量对胸腺细胞具有细胞毒性的自身抗体（天然胸腺细胞毒性自身抗体，NTA）。已发现一种单克隆NTA（NTA260）可与脑组织以及胸腺细胞的细胞表面发生反应。我们研究了NTA 260抗原在胎脑细胞原代培养物中的表达情况。固定后，NTA260强烈标记神经细胞的细胞质，但无法对活细胞进行染色。蛋白质印迹分析表明，NTA260在脑和胸腺提取物中主要识别一条约53 kDa的条带。这些发现表明，神经元NTA260抗原的分子量与胸腺细胞相似，可能是一种细胞内成分。
