Kamiya M, Miyakawa M, Ueda G, Shirai T, Arai Y
Departments of Anatomy, Juntendo University School of Medicine, Tokyo, Japan.
Neurosci Res. 1993 May;16(4):293-9. doi: 10.1016/0168-0102(93)90040-w.
On the basis of our previous report that a thymocytotoxic monoclonal autoantibody (NTA260) derived from a New Zealand Black mouse reacts with brain tissues as well as lymphoid cells, the neuronal antigen reacting with this antithymocyte antibody was characterized in the present experiment. Double staining of cultured brain cells with NTA260 and anti-tubulin antibody revealed that both staining patterns closely resembled each other. NTA260 reacted with purified tubulin molecules on Western blotting. Two-dimensional gel electrophoresis of the whole lysate of embryonic mouse brain revealed that NTA260 stained the spot corresponding to that of beta-tubulin but not that of alpha-tubulin. These results suggest that beta-tubulin is a prominent neuronal antigen recognized by NTA260.
基于我们之前的报告，即源自新西兰黑鼠的一种胸腺细胞毒性单克隆自身抗体（NTA260）可与脑组织以及淋巴细胞发生反应，在本实验中对与该抗胸腺细胞抗体发生反应的神经元抗原进行了鉴定。用NTA260和抗微管蛋白抗体对培养的脑细胞进行双重染色显示，两种染色模式彼此非常相似。在蛋白质免疫印迹分析中，NTA260与纯化的微管蛋白分子发生反应。对胚胎小鼠脑全细胞裂解物进行二维凝胶电泳显示，NTA260染色的斑点与β-微管蛋白的斑点相对应，而与α-微管蛋白的斑点不对应。这些结果表明，β-微管蛋白是NTA260识别的一种主要神经元抗原。
