来自人结肠癌细胞系的一种异常N-乙酰-β-D-己糖胺酶同工酶的特性分析。
Characterization of an unusual isoenzyme of N-acetyl-beta-D-hexosaminidase from a human colonic carcinoma cell line.
作者信息
Kimball P M, Brattain M G, White W E
出版信息
Biochem J. 1981 Jan 1;193(1):109-13. doi: 10.1042/bj1930109.
Abstract
A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line [Kimball & Brattain (1980) Cancer Res. 40, 1574-1579]. The separation and characterization of the isoenzymes of N-acetyl-beta-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.
摘要
先前从一个已建立的人结肠癌细胞系中分离出了具有增强转移能力的亚系[金博尔和布拉廷（1980年），《癌症研究》40卷，第1574 - 1579页]。本文报道了每个细胞系中N - 乙酰 - β - D - 己糖胺酶同工酶的分离和特性。亲代细胞系含有A和B同工酶。亚系缺乏A同工酶活性，含有一种非典型的B同工酶，该同工酶热不稳定，易被烷基化且分子量较低。
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本文引用的文献
1
Isolation of a cellular subpopulation from a human colonic carcinoma cell line.从人结肠癌细胞系中分离细胞亚群。
Cancer Res. 1980 May;40(5):1574-9.
2
Glycosidases in normal and dimethylhydrazine-treated rats and mice with special reference to the colonic tumours.正常及二甲基肼处理的大鼠和小鼠体内的糖苷酶，特别涉及结肠肿瘤
Br J Cancer. 1974 Jun;29(6):438-46. doi: 10.1038/bjc.1974.96.
3
Enzyme activity in invasive tumors of human breast and colon.人类乳腺和结肠浸润性肿瘤中的酶活性。
Proc Natl Acad Sci U S A. 1974 May;71(5):1833-7. doi: 10.1073/pnas.71.5.1833.
4
Partial purification of human colonic carcinoma cells by sedimentation.通过沉降法对人结肠癌细胞进行部分纯化。
Br J Cancer. 1977 Jun;35(6):850-7. doi: 10.1038/bjc.1977.128.
5
The multiple forms and kinetic differences of rat colonic beta-N-acetylhexosaminidases.大鼠结肠β-N-乙酰己糖胺酶的多种形式及动力学差异。
Biochim Biophys Acta. 1978 Apr 12;523(2):454-68. doi: 10.1016/0005-2744(78)90048-7.
6
7
Isoenzymes of beta-hexosaminidase from normal rat colon and colonic carcinoma.来自正常大鼠结肠和结肠癌的β-己糖胺酶同工酶。
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Alteration of hexosaminidase isozymes in human renal carcinoma.人肾癌中己糖胺酶同工酶的改变。
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Biochem J. 1979 Jan 1;177(1):319-30. doi: 10.1042/bj1770319.
10
The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.N-乙酰己糖胺酶与固定化伴刀豆球蛋白A的相互作用。
Biochem J. 1977 May 1;163(2):247-51. doi: 10.1042/bj1630247.
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