Alasri A, Roques C, Michel G, Cabassud C, Aptel P
Laboratoire de bacteriologie, virologie et microbiologie industrielle, Faculté des sciences pharmaceutiques, Toulouse, France.
Can J Microbiol. 1992 Jul;38(7):635-42. doi: 10.1139/m92-104.
The bactericidal properties of peracetic acid, hydrogen peroxide, chlorine, and formaldehyde were compared in vitro using a rapid micromethod. A combination of peracetic acid and hydrogen peroxide was also tested to assess interactions. The activities of these agents, which are widely used as disinfectants, were evaluated against water isolates and culture collection strains. Peracetic acid and chlorine exhibited an excellent antimicrobial activity, with a relatively rapid destruction of 10(5) bacteria/mL. The time-dependent bactericidal activities of hydrogen peroxide and formaldehyde were the lowest. The combination of peracetic acid and hydrogen peroxide, tested by a checkerboard micromethod, was found to be synergistic. The minimal bactericidal concentration was established in terms of time for a given mixture of peracetic acid and hydrogen peroxide. Determination of bactericidal concentrations showed that synergy was maintained with increasing contact time. Concentrations for minimal times of treatment by chemicals that provided interesting activities in vitro were tested for disinfection of ultrafiltration membranes. The bactericidal activities of peroxygen compounds were confirmed and synergism was maintained in working conditions. Chlorine showed a loss of efficacy when used on membranes.
采用快速微量法在体外比较了过氧乙酸、过氧化氢、氯和甲醛的杀菌特性。还测试了过氧乙酸和过氧化氢的组合以评估其相互作用。针对从水中分离出的菌株和保藏菌株，评估了这些广泛用作消毒剂的试剂的活性。过氧乙酸和氯表现出优异的抗菌活性，能相对快速地杀灭10⁵个/mL细菌。过氧化氢和甲醛的时间依赖性杀菌活性最低。通过棋盘微量法测试发现，过氧乙酸和过氧化氢的组合具有协同作用。针对给定的过氧乙酸和过氧化氢混合物，根据时间确定了最低杀菌浓度。杀菌浓度的测定表明，随着接触时间增加，协同作用得以维持。测试了在体外具有良好活性的化学试剂在最短处理时间时的浓度对超滤膜进行消毒的效果。证实了过氧类化合物的杀菌活性，并且在工作条件下协同作用得以维持。氯用于膜时显示出效力丧失。
