Stanley W S, Devine G C, Murphy B A, Seibel N, Dinndorf P
Department of Laboratory Medicine, Children's National Medical Center, Washington, D.C. 20010.
Cancer Genet Cytogenet. 1992 Sep;62(2):160-5. doi: 10.1016/0165-4608(92)90256-8.
A 15-year-old male with myelodysplastic syndrome (MDS) characterized by monosomy 7 was cytogenetically evaluated by metaphase karyotyping and fluorescence in situ hybridization (FISH) of interphase cells at six different points during the course of his disease. At diagnosis, there was complete agreement between metaphase and interphase findings. Interphase analysis alone provided important cytogenetic information on the first specimens received following intensive combination chemotherapy and bone marrow transplantation where metaphase analyses were uninformative. The detection of a minor post-treatment monosomy 7 population by interphase but not metaphase studies may have identified minimal residual disease prior to recurrence of MDS. From this longitudinal study, it is concluded that metaphase and interphase cytogenetic analyses form complementary approaches and that use of both provides greater analytical power when appropriate chromosome markers are available.
一名患有以7号染色体单体为特征的骨髓增生异常综合征（MDS）的15岁男性，在其病程中的六个不同时间点，通过中期核型分析和间期细胞荧光原位杂交（FISH）进行了细胞遗传学评估。诊断时，中期和间期结果完全一致。仅间期分析就在强化联合化疗和骨髓移植后收到的首批标本中提供了重要的细胞遗传学信息，而此时中期分析无信息价值。通过间期而非中期研究检测到治疗后少量的7号染色体单体群体，这可能在MDS复发之前就识别出了微小残留病。从这项纵向研究得出结论，中期和间期细胞遗传学分析形成互补方法，并且当有合适的染色体标志物时，两者结合使用可提供更强的分析能力。
