Ogata S, Uehara H, Chen A, Itzkowitz S H
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.
Cancer Res. 1992 Nov 1;52(21):5971-8.
Complementary DNA clones encoding four different mucin core peptides have been isolated. However, the expression of these mucin genes in the colon has not been systematically studied. The present investigation used Northern blot analysis to study the expression of MUC1, MUC2, MUC3, and MUC4 mRNA in paired normal and cancerous colonic tissues, and nine colon cancer cell lines. Results were correlated with the clinicopathological features of the tumors and with the immunohistochemical expression of several carbohydrate tumor-associated antigens that may reside on mucins. MUC1 mRNA was expressed in all colonic tissues, and levels in paired normal and cancer tissues were similar in most cases. MUC2 and MUC3 mRNAs were expressed in both normal and cancer tissues, but levels were often decreased in the cancers. MUC4 mRNA was present in normal mucosa with comparable or sometimes greater expression in cancers. There was no apparent correlation between the expression of any particular mucin gene or pattern of mucin genes and the site, stage, or histological type of tumor. In addition, the expression of mucin-associated carbohydrate antigens did not correlate with any individual mucin gene or group of mucin genes. In colon cancer cell lines all four MUC genes were expressed rather weakly or not at all. These results indicate that the human colon expresses a broad repertoire of mucin genes which are differentially regulated in malignancy. Whether this differential regulation of mucin genes affect the behavior of the tumor and results in the altered glycosylation commonly seen in these requires further investigation.
已分离出编码四种不同粘蛋白核心肽的互补DNA克隆。然而，这些粘蛋白基因在结肠中的表达尚未得到系统研究。本研究采用Northern印迹分析来研究MUC1、MUC2、MUC3和MUC4 mRNA在配对的正常和癌性结肠组织以及九种结肠癌细胞系中的表达。结果与肿瘤的临床病理特征以及几种可能存在于粘蛋白上的碳水化合物肿瘤相关抗原的免疫组化表达相关。MUC1 mRNA在所有结肠组织中均有表达，在大多数情况下，配对的正常组织和癌组织中的水平相似。MUC2和MUC3 mRNA在正常组织和癌组织中均有表达，但在癌组织中水平常常降低。MUC4 mRNA存在于正常黏膜中，在癌组织中的表达相当或有时更高。任何特定粘蛋白基因的表达或粘蛋白基因模式与肿瘤的部位、分期或组织学类型之间均无明显相关性。此外，粘蛋白相关碳水化合物抗原的表达与任何单个粘蛋白基因或粘蛋白基因组均无相关性。在结肠癌细胞系中，所有四种MUC基因的表达都相当弱或根本不表达。这些结果表明，人类结肠表达多种粘蛋白基因，它们在恶性肿瘤中受到不同的调控。粘蛋白基因的这种差异调控是否影响肿瘤的行为并导致这些肿瘤中常见的糖基化改变，还需要进一步研究。
