Packer Leisl M, Williams Stephanie J, Callaghan Shirley, Gotley David C, McGuckin Michael A
Epithelial Cancer and Mucosal Biology Laboratory, Mater Medical Research Institute, Mater Health Services, South Brisbane, Queenslands 4101, Australia.
Int J Oncol. 2004 Oct;25(4):1119-26.
Cell surface mucins are complex glycoproteins expressed on the apical membrane surface of mucosal epithelial cells. In malignant epithelial cells they are thought to influence cell adhesion, and are clinical targets for tumor immunotherapy and serum tumor marker assays. We have compared expression of MUC1, MUC3, MUC4, MUC11, MUC12 and MUC13 mRNA in epithelial cancers and/or cell lines with non-malignant tissues. In non-malignant tissues, MUC3, 4, 11, 12 and 13 were expressed at highest levels in gastrointestinal tissues, whereas MUC1 was more widely distributed. Significant down-regulation of the MUC4, MUC12 and MUC13 genes was observed in colonic cancers compared with normal tissue, whereas MUC1 was upregulated. In rectal cancers, levels of all six mucin genes were not significantly different to those in normal rectal tissues. Both MUC1 and MUC4 were down-regulated in gastric cancers, whereas cancer and normal tissue levels were similar for MUC3, 11, 12 and 13. In esophageal cancers there was a general trend toward higher levels than in normal tissue for MUC1, 3, 12 and 13. In ovarian cancers MUC1 levels were very high, whereas only low levels of all other mucins were observed. We also report expression in renal cell carcinomas, bladder carcinomas and breast cancer cell lines. The reported expression profiles of the cell surface mucin gene family will help direct biological and clinical studies of these molecules in mucosal biology, and in malignant and inflammatory diseases of epithelial tissues.
细胞表面黏蛋白是在黏膜上皮细胞顶端膜表面表达的复杂糖蛋白。在恶性上皮细胞中，它们被认为会影响细胞黏附，并且是肿瘤免疫治疗和血清肿瘤标志物检测的临床靶点。我们比较了上皮癌和/或细胞系与非恶性组织中MUC1、MUC3、MUC4、MUC11、MUC12和MUC13 mRNA的表达情况。在非恶性组织中，MUC3、4、11、12和13在胃肠道组织中表达水平最高，而MUC1分布更广泛。与正常组织相比，在结肠癌中观察到MUC4、MUC12和MUC13基因显著下调，而MUC1上调。在直肠癌中，所有六个黏蛋白基因的水平与正常直肠组织相比无显著差异。在胃癌中，MUC1和MUC4均下调，而MUC3、11、12和13在癌组织和正常组织中的水平相似。在食管癌中，MUC1、3、12和13的水平总体上有高于正常组织的趋势。在卵巢癌中，MUC1水平非常高，而其他所有黏蛋白仅观察到低水平表达。我们还报告了肾细胞癌、膀胱癌和乳腺癌细胞系中的表达情况。所报道的细胞表面黏蛋白基因家族的表达谱将有助于指导这些分子在黏膜生物学以及上皮组织恶性和炎症性疾病中的生物学和临床研究。
