Seregni E, Botti C, Lombardo C, Cantoni A, Bogni A, Cataldo I, Bombardieri E
Nuclear Medicine Department, Istituto Nazionale Tumori, Milano, Italy.
Anticancer Res. 1996 Jul-Aug;16(4B):2209-13.
This work evaluates the expression in lung cancer of the most well characterized mucin genes (MUC1, MUC2, MUC3) and of the recently described MUC4 in lung tissues, to check a correlation between the expression of any particular gene and this tumor. Hybridization with synthetic oligonucleotides obtained from a part of the sequences of MUC1, MUC2, MUC3 and MUC4, was performed on blotted RNA from 18 lung cancer tissue specimens and from 10 normal tissues samples taken, when possible, from the normal lung counterpart. By means of Northern blot analysis MUC1 revealed to be the most expressed mucin gene in lung cancer, followed by MUC4; by contrast, the expression of MUC2 and MUC3 was almost undetectable in all cancer specimens. The intensity of expression of MUC1 and MUC4 was always superior in cancer tissue than in the normal counterpart. As expected, the highest reactivity for MUC1 and MUC4 expression was observed mainly in the adenocarcinoma histotype which is mucin secreting. These findings represent a contribution to the study of mucin gene pattern in lung cancer, and, in particular, indicate that MUC4, in association with the MUC1 gene, seems to be strongly expressed in this neoplastic disease.
本研究评估了特征最明确的黏蛋白基因（MUC1、MUC2、MUC3）以及最近描述的MUC4在肺癌组织中的表达情况，以检验任何特定基因的表达与该肿瘤之间的相关性。使用从MUC1、MUC2、MUC3和MUC4部分序列获得的合成寡核苷酸，对18份肺癌组织标本和10份正常组织标本（尽可能取自相应的正常肺组织）的印迹RNA进行杂交。通过Northern印迹分析发现，MUC1是肺癌中表达最多的黏蛋白基因，其次是MUC4；相比之下，在所有癌症标本中几乎检测不到MUC2和MUC3的表达。MUC1和MUC4在癌组织中的表达强度始终高于正常组织。正如预期的那样，MUC1和MUC4表达的最高反应性主要在分泌黏蛋白的腺癌组织学类型中观察到。这些发现为肺癌黏蛋白基因模式的研究做出了贡献，特别是表明MUC4与MUC1基因一起，似乎在这种肿瘤疾病中强烈表达。
