Recio L, Sisk S, Pluta L, Bermudez E, Gross E A, Chen Z, Morgan K, Walker C
Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
Cancer Res. 1992 Nov 1;52(21):6113-6.
Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of > or = 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of the rat p53 complementary DNA containing the evolutionarily conserved regions II-V was directly sequenced from each tumor. Point mutations in the p53 complementary DNA sequence were found in 5 of 11 of the tumors analyzed. These data demonstrate p53 point mutations in formaldehyde-induced squamous cell carcinomas and indicate a common alteration in certain rat and human squamous cell carcinomas of the respiratory tract.
在大鼠慢性吸入浓度≥10 ppm的甲醛后，甲醛会诱发鼻腔鳞状细胞癌。我们使用DNA序列分析，检查了11个原发性甲醛诱导肿瘤中肿瘤抑制基因p53的互补DNA是否发生突变。从每个肿瘤中直接对包含进化保守区域II - V的大鼠p53互补DNA的聚合酶链反应扩增片段进行测序。在分析的11个肿瘤中有5个发现了p53互补DNA序列中的点突变。这些数据证明了甲醛诱导的鳞状细胞癌中存在p53点突变，并表明在某些大鼠和人类呼吸道鳞状细胞癌中存在共同的改变。
