Owen F L, Stux S V, Nisonoff A
J Immunol. 1977 Mar;118(3):909-19.
Substantial percentages of T-enriched spleen lymphocytes or thymocytes of inbred rats were found to form rosettes with the RBC of homologous strains. When an excess of RBC was used, essentially all of the rosette-forming subpopulation of lymphocytes was removed when the rosettes were separated by centrifugation. After depletion of the lymphocytes, reactive with RBC of one strain, most of the lymphocytes reactive with RBC of other strains could be recovered in the supernatant. A very large percentage of lymphocytes of the BN strain formed rosettes when a mixture of the RBC of five other strains was tested; the percentage was, however, somewhat lower than that predicted on the basis of complete additivity. Rosettes dissociated when warmed to 37 degrees C. The lymphocytes recovered were unable to form rosettes again. In nearly all instances, the subpopulation that formed rosettes with RBC of a given strain included essentially all of the lymphocytes that acted as aggressors against peripheral leukocytes or mytomycin C-treated thymocytes of that strain; the lymphocytes in the supernatant always retained activity as aggressors against the leukocytes of one or more other strains and retained their responsiveness to PHA. Lymphocytes recovered from rosettes, by warming to 37 degrees C, were highly reactive as aggressors in the MLR against the strain providing the RBC. Varying degrees of reactivity were noted against leukocytes of other strains.
发现近交系大鼠富含T细胞的脾淋巴细胞或胸腺细胞中有相当比例能与同源品系的红细胞形成花环。当使用过量红细胞时，通过离心分离花环后，基本上所有形成花环的淋巴细胞亚群都会被去除。在用一种品系的红细胞处理淋巴细胞耗尽后，与其他品系红细胞反应的大多数淋巴细胞可在上清液中回收。当测试其他五个品系的红细胞混合物时，BN品系的很大比例的淋巴细胞形成了花环；然而，该比例略低于基于完全加性预测的比例。花环在37℃加热时会解离。回收的淋巴细胞无法再次形成花环。几乎在所有情况下，与给定品系的红细胞形成花环的亚群基本上包括所有对该品系的外周白细胞或丝裂霉素C处理的胸腺细胞起攻击作用的淋巴细胞；上清液中的淋巴细胞始终保留对一种或多种其他品系白细胞的攻击活性，并保留其对PHA的反应性。通过在37℃加热从花环中回收的淋巴细胞在针对提供红细胞的品系的混合淋巴细胞反应中作为攻击细胞具有高反应性。对其他品系的白细胞观察到不同程度的反应性。
