Galili U, Schlesinger M
J Immunol. 1976 Sep;117(3):730-5.
The aim of the present study was to determine whether activation of human T-lymphocytes affects their interaction with sheep red blood cells (SRBC). Less than 3% of the E-rosettes formed by freshly isolated peripheral blood lymphocytes (PBL) and SRBC are stable and do not disintegrate after incubation at 37 degrees C. In contrast, about 30% of PBL kept in culture for 5 days in the presence of mitomycin C-treated allogeneic lymphocytes were found to form stable E-rosettes. Whereas no rosettes were formed by freshly isolated PBL incubated with human red blood cells at 24 degrees C, 15% of the cells recovered from mixed lymphocyte reactions (MLR) formed such rosettes. When responder PBL were maintained in culture in the absence of allogeneic stimuli the proportion of cells forming stable E-rosettes depended on the serum present in the medium. Less than 5% of the responder cells kept in medium containing human serum or in serum-free medium formed stable E-rosettes, whereas 18% of the cells maintained in medium containing fetal calf serum formed stable E-rosettes. The proportion of cells forming stable E-rosettes increased before any increase in DNA synthesis was detectable in MLR. Indeed, a high proportion of cells forming stable E-rosettes appeared in MLR taking place in serum-free medium, without any accompanying increase of DNA synthesis. Depletion of cells forming EAC'-rosettes from responder PBL increased the proportion of cells forming stable E-rosettes in MLR. Exposure of the cells recovered in MLR to specific anti-T sera inhibited the formation of both stable and regular E-rosettes. Exposure of the cells recovered in MLR to anti-Ig serum had no effect on the formation of regular rosettes. Anti-Ig serum strongly inhibited the formation of stable E-rosettes by cells grown in medium containing human serum, but had no effect on the formation of stable E-rossettes by cells grown in either serum-free medium or in serum containing fetal calf serum. It is concluded that activated human T lymphocytes are characterized by their capacity to form stable E-rosettes, resistant to incubation at 37 degrees C, and by their capacity to acquire an immunoglobulin coat, possibly by binding immunoglobulin molecules present in their environment.
本研究的目的是确定人类T淋巴细胞的激活是否会影响其与绵羊红细胞（SRBC）的相互作用。新鲜分离的外周血淋巴细胞（PBL）与SRBC形成的E花环中，不到3%是稳定的，在37℃孵育后不会解体。相比之下，在丝裂霉素C处理的同种异体淋巴细胞存在下培养5天的PBL中，约30%被发现能形成稳定的E花环。新鲜分离的PBL在24℃与人红细胞孵育时不形成花环，但从混合淋巴细胞反应（MLR）中回收的细胞中有15%形成了此类花环。当反应性PBL在无同种异体刺激的情况下在培养中维持时，形成稳定E花环的细胞比例取决于培养基中存在的血清。在含人血清的培养基或无血清培养基中培养的反应性细胞中，形成稳定E花环的细胞不到5%，而在含胎牛血清的培养基中培养的细胞中有18%形成了稳定的E花环。在MLR中可检测到DNA合成增加之前，形成稳定E花环的细胞比例就已增加。事实上，在无血清培养基中进行的MLR中出现了高比例形成稳定E花环的细胞，且DNA合成没有任何相应增加。从反应性PBL中去除形成EAC'-花环的细胞会增加MLR中形成稳定E花环的细胞比例。将MLR中回收的细胞暴露于特异性抗T血清会抑制稳定和常规E花环的形成。将MLR中回收的细胞暴露于抗Ig血清对常规花环的形成没有影响。抗Ig血清强烈抑制在含人血清的培养基中生长的细胞形成稳定E花环，但对在无血清培养基或含胎牛血清的血清中生长的细胞形成稳定E花环没有影响。结论是，活化的人类T淋巴细胞的特征在于它们形成稳定E花环的能力，这种花环在37℃孵育时具有抗性，以及它们获得免疫球蛋白包被的能力，可能是通过结合其环境中存在的免疫球蛋白分子。
