Hosoda H, Fukuda K, Goto J
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Chem Pharm Bull (Tokyo). 1992 May;40(5):1325-6. doi: 10.1248/cpb.40.1325.
The p-nitrophenyl ester method was assessed as an enzyme labeling technique. The active ester of a carboxylated testosterone derivative was treated with alkaline phosphatase and glucose oxidase to give labeled antigens, using various molar ratios of steroid to enzyme. Satisfactory immunoreactivities with an anti-testosterone antibody in an enzyme immunoassay system were obtained with the labeled antigens prepared at pH 8.5 by the use of molar ratios higher than 30 and 10, respectively, in the alkaline phosphatase and glucose oxidase labelings.
对硝基苯酯法作为一种酶标记技术进行了评估。将羧化睾酮衍生物的活性酯与碱性磷酸酶和葡萄糖氧化酶反应，使用不同的类固醇与酶的摩尔比来制备标记抗原。在酶免疫分析系统中，通过在pH 8.5下分别使用高于30和10的摩尔比进行碱性磷酸酶和葡萄糖氧化酶标记制备的标记抗原，与抗睾酮抗体获得了令人满意的免疫反应性。
