Hosoda H, Fukuda K, Takahashi N, Goto J
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Chem Pharm Bull (Tokyo). 1992 Jan;40(1):294-5. doi: 10.1248/cpb.40.294.
A thermostable glucose-6-phosphate dehydrogenase was assessed as a label enzyme in a testosterone enzyme immunoassay system. Enzyme labeling of a steroid with the enzyme was carried out by the N-succinimidyl ester method. A dose-response curve with a satisfactory sensitivity could be obtained by the use of the enzyme-labeled antigen. This enzyme should be useful in hapten enzyme immunoassays because of its relatively high stability.
一种热稳定的葡萄糖-6-磷酸脱氢酶在睾酮酶免疫分析系统中被评估为标记酶。用该酶对类固醇进行酶标记是通过N-琥珀酰亚胺酯法进行的。使用酶标记抗原有望获得具有令人满意灵敏度的剂量反应曲线。由于其相对较高的稳定性，这种酶在半抗原酶免疫分析中应会很有用。
