• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在类固醇酶免疫分析系统中，葡萄糖氧化酶标记抗原的免疫反应性意外丧失。

Unexpected loss of immunoreactivity of a glucose oxidase-labeled antigen in a steroid enzyme immunoassay system.

作者信息

Hosoda H, Itoh M, Tsukamoto R, Nambara T

出版信息

Chem Pharm Bull (Tokyo). 1989 Mar;37(3):859-60. doi: 10.1248/cpb.37.859.

DOI:10.1248/cpb.37.859
PMID:2665963
Abstract

Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.

摘要

使用单克隆抗体在酶免疫分析系统中检测葡萄糖氧化酶标记的11-脱氧皮质醇的免疫反应性。抗原通过N-琥珀酰亚胺酯法进行标记。结果发现，标记物与抗类固醇抗体的结合亲和力在储存期间显著降低；半衰期约为4.5天。

相似文献

1
Unexpected loss of immunoreactivity of a glucose oxidase-labeled antigen in a steroid enzyme immunoassay system.在类固醇酶免疫分析系统中，葡萄糖氧化酶标记抗原的免疫反应性意外丧失。
Chem Pharm Bull (Tokyo). 1989 Mar;37(3):859-60. doi: 10.1248/cpb.37.859.
2
Sensitivity of steroid enzyme immunoassays. Comparison of four label enzymes in an assay system using a monoclonal anti-steroid antibody.类固醇酶免疫测定的灵敏度。在使用单克隆抗类固醇抗体的测定系统中对四种标记酶的比较。
Chem Pharm Bull (Tokyo). 1989 Jul;37(7):1834-7. doi: 10.1248/cpb.37.1834.
3
Enzyme labeling of steroids by the N-succinimidyl ester method. Preparation of glucose oxidase-labeled antigen for use in enzyme immunoassay.通过N-琥珀酰亚胺酯法对类固醇进行酶标记。制备用于酶免疫测定的葡萄糖氧化酶标记抗原。
Chem Pharm Bull (Tokyo). 1987 Dec;35(12):4856-61. doi: 10.1248/cpb.35.4856.
4
Enzyme labeling in steroid enzyme immunoassays. The p-nitrophenyl ester method for alkaline phosphatase and glucose oxidase labelings.类固醇酶免疫分析中的酶标记。用于碱性磷酸酶和葡萄糖氧化酶标记的对硝基苯酯法。
Chem Pharm Bull (Tokyo). 1992 May;40(5):1325-6. doi: 10.1248/cpb.40.1325.
5
Enzyme labeling in steroid enzyme immunoassays. Comparison of the p-nitrophenyl ester and N-succinimidyl ester methods.类固醇酶免疫测定中的酶标记。对硝基苯酯法和N-琥珀酰亚胺酯法的比较。
Chem Pharm Bull (Tokyo). 1991 Sep;39(9):2373-7. doi: 10.1248/cpb.39.2373.
6
Production of monoclonal antibodies against glucose oxidase, alkaline phosphatase and peroxidase. Their application in a highly sensitive antigen spot microassay.
J Immunol Methods. 1987 Aug 3;101(2):251-9. doi: 10.1016/0022-1759(87)90157-8.
7
Use of the thermostable glucose-6-phosphate dehydrogenase as a label enzyme in steroid enzyme immunoassays.热稳定葡萄糖-6-磷酸脱氢酶在类固醇酶免疫测定中作为标记酶的应用。
Chem Pharm Bull (Tokyo). 1992 Jan;40(1):294-5. doi: 10.1248/cpb.40.294.
8
An unlabeled antibody method using glucose oxidase-antiglucose oxidase complexes (GAG): a sensitive alternative to immunoperoxidase for the detection of tissue antigens.一种使用葡萄糖氧化酶 - 抗葡萄糖氧化酶复合物（GAG）的未标记抗体方法：用于检测组织抗原的免疫过氧化物酶的灵敏替代方法。
J Histochem Cytochem. 1982 Jan;30(1):27-34. doi: 10.1177/30.1.7033369.
9
[Antigen enzyme coupling: a few results (author's transl)].
Ann Biol Clin (Paris). 1978;36(4):397-400.
10
Enzyme labeling of steroids by the N-succinimidyl ester method. Preparation of alkaline phosphatase-labeled antigen for use in enzyme immunoassay.
Chem Pharm Bull (Tokyo). 1985 Dec;33(12):5393-8. doi: 10.1248/cpb.33.5393.