Nagi N, Ohno N, Tanaka S, Aketagawa J, Shibata Y, Yadomae T
Lab. Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Chem Pharm Bull (Tokyo). 1992 Jun;40(6):1532-6. doi: 10.1248/cpb.40.1532.
Solubilization of limulus test reactive materials from Candida was examined in the presence or absence of phagocytic cells. Solubilized limulus test reactive materials (LTRM) were detected in culture supernatant, and hot water and sodium hydroxide extracts of the acetone dried cells of Candida parapsilosis. Suspensions of Candida cells also reacted with limulus test, and LTRM were released from the acetone dried cells by serum treatment. After treatment of the acetone dried cells with polymorphonuclear leucocytes (PMN) or macrophages (M phi), a significant amount of LTRM was solubilized. Significant amounts of LTRM were also released by PMN during treatment of live and growing C. parapsilosis. The reactivity of LTRM was completely inhibited by the addition of excess amount of purified (1----3)-beta-D-glucan, suggesting LTRM from Candida cells as described above would contain (1----3)-beta-D-glucan. These results suggested that LTRM during fungal infection would come from the extracellular water soluble polysaccharide fraction as well as the insoluble cell wall fraction solubilized by the action of phagocytes.
在有或没有吞噬细胞存在的情况下，研究了从念珠菌中溶解鲎试剂反应性物质的情况。在培养上清液以及近平滑念珠菌丙酮干燥细胞的热水提取物和氢氧化钠提取物中检测到了溶解的鲎试剂反应性物质（LTRM）。念珠菌细胞悬液也与鲎试剂发生反应，并且通过血清处理从丙酮干燥细胞中释放出LTRM。用多形核白细胞（PMN）或巨噬细胞（M phi）处理丙酮干燥细胞后，大量LTRM被溶解。在活的且正在生长的近平滑念珠菌处理过程中，PMN也释放出大量LTRM。加入过量的纯化（1→3）-β-D-葡聚糖可完全抑制LTRM的反应性，这表明上述来自念珠菌细胞的LTRM将含有（1→3）-β-D-葡聚糖。这些结果表明，真菌感染期间的LTRM将来自细胞外水溶性多糖部分以及通过吞噬细胞作用溶解的不溶性细胞壁部分。
