GIBBS S P
J Cell Biol. 1962 Nov;15(2):343-61. doi: 10.1083/jcb.15.2.343.
When dark-grown cells of Ochromonas danica are placed in the light, the amount of chlorophyll a per cell increases 82-fold; the content of carotenoid pigment, 24-fold. Concomitantly with this increase in chlorophyll and carotenoid pigment, the small proplastid of dark-grown cells develops into a large lamellate chloroplast. During the first 12 hours in the light, vesicles appear within the loose clusters of dense chloroplast granules, enlarge, align themselves into rows (plates in three dimensions), and fuse into discs. Double discs may form from the more or less simultaneous fusion of two adjacent plates of vesicles or by the addition of vesicles to an already formed single disc. Three-disc bands arise by the addition of a disc to an already formed two-disc band through the approach and fusion of more vesicles. After 24 hours in the light, most of the chloroplast bands contain three discs, but the chloroplasts are still small. After 48 hours in the light, almost all the cells contain full-sized chloroplasts with a full complement of three-disc bands. However, at this time the amount of chlorophyll a and carotenoid pigment is only one-half of maximum. During the next 3 days in the light, as the number of chlorophyll and carotenoid molecules per chloroplast approximately doubles, there is a compression of the discs in each band (from 180 to 130 A) and a precise alignment of their membranes. Changes also occur in the nucleus when dark-grown cells are placed in the light. There is an increase in the number of small nucleolar bodies, many of which lie directly against the nuclear envelope, and in a few cells a dense mass of granules is seen between the two membranes of the nuclear envelope.
当将黑暗中生长的丹麦赭球藻细胞置于光照下时，每个细胞中叶绿素a的含量增加82倍；类胡萝卜素色素的含量增加24倍。伴随着叶绿素和类胡萝卜素色素的这种增加，黑暗中生长的细胞中的小前质体发育成大的片状叶绿体。在光照的最初12小时内，囊泡出现在密集的叶绿体颗粒的松散簇中，增大，排列成排（三维的板状），并融合成圆盘。双圆盘可能由两个相邻的囊泡板或多或少同时融合形成，或者通过向已经形成的单个圆盘添加囊泡而形成。三圆盘带是通过更多囊泡的靠近和融合，将一个圆盘添加到已经形成的双圆盘带上而产生的。光照24小时后，大多数叶绿体带含有三个圆盘，但叶绿体仍然很小。光照48小时后，几乎所有细胞都含有全尺寸的叶绿体，且具有完整的三圆盘带。然而，此时叶绿素a和类胡萝卜素色素的含量仅为最大值的一半。在接下来光照的3天里，随着每个叶绿体中叶绿素和类胡萝卜素分子的数量大约翻倍，每个带中的圆盘会压缩（从180埃到130埃），并且它们的膜会精确排列。当黑暗中生长的细胞置于光照下时，细胞核也会发生变化。小核仁体的数量增加，其中许多直接靠在核膜上，并且在少数细胞中，在核膜的两层膜之间可见一团密集的颗粒。
