从海胆的卵和胚胎中分离细胞核。
The isolation of nuclei from eggs and embryos of the sea urchin.
作者信息
HINEGARDNER R T
出版信息
J Cell Biol. 1962 Dec;15(3):503-8. doi: 10.1083/jcb.15.3.503.
Abstract
NUCLEI CAN BE ISOLATED FROM THE EGGS AND EMBRYOS OF THE SEA URCHIN BY A FOUR STEP METHOD
(1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used.
摘要
海胆的细胞核可通过一种四步法从其卵和胚胎中分离出来
（1）用葡萄糖溶液洗涤直至细胞变弱；（2）在0.002M Mg(++)溶液中进行细胞溶解；（3）通过搅拌使已溶解细胞匀浆化；（4）在分层蔗糖梯度上进行离心，将细胞核与匀浆的其余部分分离。分离出的细胞核的污染率低于1%。产量根据所使用的发育阶段在20%至80%之间变化。
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