Fraser M, Carter A M, Challis J R, McDonald T J
Department of Ob/Gyn, Lawson Research Institute, University of Western Ontario, London, Canada.
Endocrinology. 1992 Oct;131(4):2033-5. doi: 10.1210/endo.131.4.1396347.
Using antisera directed towards the C-terminal region of gastrin releasing peptide (GRP), significant quantities of GRP-like immunoreactivity (GRPLI) were detected in ovine amniotic fluid and in the fetal and maternal circulations. The highest GRPLI levels were found in amniotic fluid (2135 +/- 829 fmol/ml, n = 12; mean +/- SEM), followed by those in ovine fetal (604 +/- 267 fmol/ml, n = 13) and maternal plasma (229 +/- 89 fmol/ml, n = 13). On gel filtration chromatography, the predominant GRPLI form in each fluid eluted in an identical position consistent with the entity being of apparently larger molecular size than porcine GRP1-27. Certain fetal plasma samples contained a second GRPLI peak eluting at the void volume. Hence, during ovine pregnancy a GRPLI entity circulates in fetal and maternal plasma; the entity is of apparently larger molecular size than GRP1-27 but contains a structure immunologically indistinguishable from the bioactive c-terminal region of GRP1-27. Given the recognized bioactivities of GRP, this entity may be an important hormone during ovine fetal life.
使用针对胃泌素释放肽（GRP）C末端区域的抗血清，在绵羊羊水以及胎儿和母体循环中检测到大量的GRP样免疫反应性（GRPLI）。在羊水中发现的GRPLI水平最高（2135±829 fmol/ml，n = 12；平均值±标准误），其次是绵羊胎儿（604±267 fmol/ml，n = 13）和母体血浆（229±89 fmol/ml，n = 13）中的水平。在凝胶过滤色谱中，每种液体中主要的GRPLI形式在相同位置洗脱，这表明该物质的分子大小明显大于猪GRP1-27。某些胎儿血浆样本在空体积处含有第二个GRPLI峰。因此，在绵羊妊娠期间，一种GRPLI物质在胎儿和母体血浆中循环；该物质的分子大小明显大于GRP1-27，但含有一种在免疫学上与GRP1-27的生物活性C末端区域无法区分的结构。鉴于GRP已被认可的生物活性，这种物质可能是绵羊胎儿期的一种重要激素。
