ASHERSON G L, DUMONDE D C
Immunology. 1964 Jan;7(1):1-8.
The injection of rat liver and rat brain in Freund's complete adjuvant into rabbits led to the production of complement fixing autoantibodies against rabbit tissue. The autoantibody response following the injection of rabbit liver and brain was much smaller. The autoantibody response to rat liver occurred even when Freund's incomplete adjuvant was used. The injection of rabbit liver in Freund's complete adjuvant into rabbits which had produced autoantibody after the injection of rat liver did not increase the level of autoantibody to rabbit liver. In most, but not all, rabbits the natural antibody found in the sera taken before immunization was destroyed by heating the sera at 65°. After immunization with rat liver in Freund's complete adjuvant the levels of serum antibody to rabbit liver stable at 56° rose by day 5; however, autoantibody stable at 65° was not detectable until between days 7 and 14.
将弗氏完全佐剂中的大鼠肝脏和大鼠脑注射到兔子体内,会导致产生针对兔组织的补体结合自身抗体。注射兔肝脏和兔脑后产生的自身抗体反应要小得多。即使使用弗氏不完全佐剂,对大鼠肝脏的自身抗体反应仍会发生。将弗氏完全佐剂中的兔肝脏注射到在注射大鼠肝脏后已产生自身抗体的兔子体内,并未提高对兔肝脏的自身抗体水平。在大多数(但不是所有)兔子中,免疫前血清中发现的天然抗体在65°加热血清后被破坏。在用弗氏完全佐剂中的大鼠肝脏免疫后,56°稳定的血清抗兔肝脏抗体水平在第5天上升;然而,65°稳定的自身抗体直到第7天至第14天之间才检测到。