大鼠肝细胞溶质中膳食视黄醇、α-生育酚与脂质过氧化之间的关系。

Relationship between dietary retinol and alpha-tocopherol and lipid peroxidation in rat liver cytosol.

作者信息

Melin A M, Carbonneau M A, Thomas M J, Maviel M J, Perromat A, Clerc M

机构信息

Laboratoire de Biochimie Médicale A, Université de Bordeaux II, France.

出版信息

Food Addit Contam. 1992 Jan-Feb;9(1):1-9. doi: 10.1080/02652039209374042.

DOI:10.1080/02652039209374042

PMID:1397387

Abstract

The effects of retinol and alpha-tocopherol-deficient and supplemented diets on the cytosolic concentration of thiobarbituric acid reactive substances (TBARS) in rat liver have been studied. Physiological lipoperoxidation (LPO) was observed in liver cytosol of control rats (TBARS = 0.315 +/- 0.034 nmol of MDA equivalents/mg of liver cytosolic proteins). In retinol-deficient diets there was a decrease in retinolaemia and the absence of retinol in liver cytosol while cytosolic TBARS increased significantly (P less than 0.001). Vitamin E was not found in cytosolic fractions, except in alpha-tocopherol-supplemented diet rats. alpha-Tocopherol-deficient diets induced an absence of vitamin E in the serum and cytosolic TBARS were increased compared to controls (P less than 0.001). Supplementation of the diet with retinol and alpha-tocopherol or both in combination induced a significant decrease in liver cytosolic TBARS (P less than 0.001). Finally the combination of low dietary supplementation with retinol and alpha-tocopherol (ten times the normal diet each) induced the maximum anti-LPO effect.

摘要

研究了缺乏和补充视黄醇及α-生育酚的饮食对大鼠肝脏细胞溶质中硫代巴比妥酸反应性物质（TBARS）浓度的影响。在对照大鼠的肝脏细胞溶质中观察到生理性脂质过氧化（LPO）（TBARS = 0.315±0.034 nmol丙二醛当量/毫克肝脏细胞溶质蛋白）。在缺乏视黄醇的饮食中，血液视黄醇水平降低，肝脏细胞溶质中不存在视黄醇，而细胞溶质TBARS显著增加（P<0.001）。除了补充α-生育酚的饮食组大鼠外，在细胞溶质组分中未发现维生素E。缺乏α-生育酚的饮食导致血清中缺乏维生素E，与对照组相比，细胞溶质TBARS增加（P<0.001）。在饮食中补充视黄醇和α-生育酚或两者组合可导致肝脏细胞溶质TBARS显著降低（P<0.001）。最后，低剂量补充视黄醇和α-生育酚（各自为正常饮食的十倍）的组合诱导了最大的抗脂质过氧化作用。