SBARRA A J, SHIRLEY W, BAUMSTARK J S
J Bacteriol. 1963 Feb;85(2):306-13. doi: 10.1128/jb.85.2.306-313.1963.
Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was found that the addition of compounds such as KCl, NaCl, sodium or potassium malonate, and K(2)SO(4) to the phagocytic system inhibited phagocytosis. The increased salt concentration specifically inhibited the respiratory activity associated with phagocytosis. The endogenous respiration of the leukocytes was unaffected. Glycolysis and the increased flow of glucose through the hexose monophosphate pathway were also inhibited by the elevated concentration of salts. In addition, cells exposed to high salt concentrations appeared to be reduced in size as compared with normal cells. The inhibition can be reversed by lowering the salt concentration. It was suggested that the increased osmotic pressure of the system was responsible for the inhibition.
斯巴拉，安东尼·J.（马萨诸塞州波士顿圣玛格丽特医院），威廉·雪莉，以及约翰·S.鲍姆施塔克。渗透压对吞噬作用的影响。《细菌学杂志》85:306 - 313。1963年。——研究了多种不同化合物对吞噬作用的影响。通过形态学和生物化学方法监测吞噬作用。发现向吞噬系统中添加氯化钾、氯化钠、丙二酸钠或丙二酸钾以及硫酸钾等化合物会抑制吞噬作用。盐浓度的增加特异性地抑制了与吞噬作用相关的呼吸活性。白细胞的内源性呼吸未受影响。糖酵解以及通过己糖磷酸途径的葡萄糖流量增加也受到盐浓度升高的抑制。此外，与正常细胞相比，暴露于高盐浓度下的细胞似乎尺寸减小。降低盐浓度可使抑制作用逆转。有人提出系统渗透压的增加是造成这种抑制的原因。
