ELSBACH P, SCHWARTZ I L
J Gen Physiol. 1959 May 20;42(5):883-98. doi: 10.1085/jgp.42.5.883.
Rabbit polymorphonuclear leukocytes obtained from peritoneal exudates, incubated at 37 degrees C. following exposure to 4 degrees C., actively reaccumulate potassium while little or no net extrusion of sodium takes place. Preventing the utilization of oxidative metabolism with potassium cyanide, 2,4-dinitrophenol, or a nitrogen atmosphere does not inhibit the recovery process. Inhibitors blocking anaerobic glycolysis (sodium iodoacetate and sodium fluoride in low concentrations) completely abolish the capacity to reaccumulate potassium and cause a further dissipation of the sodium and potassium gradients. Water movements have been shown to be secondary to cation shifts. It is postulated that separate transport mechanisms exist for sodium and potassium and that the process of potassium reaccumulation relies on anaerobic glycolysis as a source of energy.
从腹膜渗出液中获取的兔多形核白细胞，在暴露于4℃后于37℃孵育，会积极地重新积累钾，而钠的净排出很少或没有发生。用氰化钾、2,4 -二硝基苯酚或氮气环境阻止氧化代谢的利用并不会抑制恢复过程。阻断无氧糖酵解的抑制剂（低浓度的碘乙酸钠和氟化钠）会完全消除重新积累钾的能力，并导致钠和钾梯度的进一步消散。已表明水的移动是阳离子转移的继发现象。据推测，钠和钾存在独立的转运机制，并且钾的重新积累过程依赖无氧糖酵解作为能量来源。
