通过共价色谱法纯化组蛋白F3。

Purification of histone F3 by covalent chromatography.

作者信息

Oster O, Buchlow G

出版信息

Z Naturforsch C Biosci. 1977 Jan-Feb;32(1-2):12-4.

Abstract

The arginine-rich histone F3 has been purified by covalent affinity chromatography. By the use of activated Thiol-Sepharose 4B for the purification of cysteine containing histone F3 a highly purified protein was obtained. The simple purification procedure offers the opportunity to get larger amounts of pure histone F3 within short time.

摘要

富含精氨酸的组蛋白F3已通过共价亲和层析法纯化。通过使用活化的硫醇-琼脂糖凝胶4B来纯化含半胱氨酸的组蛋白F3，获得了一种高度纯化的蛋白质。这种简单的纯化方法提供了在短时间内获得大量纯组蛋白F3的机会。