来自大鼠肝脏和胸腺细胞核的组蛋白f3中的磷酸基团和硫醇基团。
Phosphate and thiol groups in histone f3 from rat liver and thymus nuclei.
作者信息
Ord M G, Stocken L A
出版信息
Biochem J. 1967 Feb;102(2):631-6. doi: 10.1042/bj1020631.
Abstract
- Differences in washing procedures and conditions of dialysis affected the type of histone f3 obtained from rat liver and thymus nuclei. 2. Sulphur-rich preparations of f3 contained phosphate and had SH+SS: phosphorus ratio 1:2. 3. Sulphur-rich f3 from livers of [(14)C]adenine-labelled rats gave counts indicating a phosphorus: adenine ratio 1:1. 4. Evidence was presented that histones f3 and f1 exist in nuclei as families of proteins, differing with respect to their sulphur or phosphorus content or both. 5. The significance of the different states of phosphorylation and oxidation of histones f1 and f3 was discussed.
摘要
- 透析的洗涤程序和条件差异会影响从大鼠肝脏和胸腺细胞核中获得的组蛋白f3的类型。2. 富含硫的f3制剂含有磷酸盐，且SH+SS与磷的比例为1:2。3. 来自[(14)C]腺嘌呤标记大鼠肝脏的富含硫的f3产生的计数表明磷与腺嘌呤的比例为1:1。4. 有证据表明，组蛋白f3和f1在细胞核中以蛋白质家族的形式存在，它们在硫或磷含量或两者方面存在差异。5. 讨论了组蛋白f1和f3不同磷酸化和氧化状态的意义。
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