Borzini P, Riva M
Centro Trasfusionale, Ospedale San Gerardo, Monza, Italy.
Haematologica. 1992 Jan-Feb;77(1):89-91.
Fresh washed platelets are commonly used as target cells for the detection of platelet reactive antibodies. The use of stored platelets would allow a faster execution of the tests and a rapid selection of phenotyped cells.
Glass-adherent platelet monolayers were freeze stored. The antigen-antibody reaction between platelet antigens and platelet reactive allo- or autoantibodies was revealed by a modified antiglobulin test, using colloidal gold-labeled anti-human IgG. Immunogold staining (IGS) was compared with the platelet suspension immunofluorescence test (PSIFT) and with the solid-phase red cell adherence assay (SPRCA). The reactivity of fresh and freeze-stored platelets was compared.
IGS proved to be slightly less sensitive (10%) than PSIFT and SPRCA: The results obtained with fresh and freeze-stored cells were scored identically.
The immunogold staining assay on freeze-stored platelets seems to be a convenient technical approach for platelet serology.
新鲜洗涤血小板通常用作检测血小板反应性抗体的靶细胞。使用储存的血小板将使检测能够更快地进行，并能快速选择表型细胞。
将玻璃粘附血小板单层进行冷冻保存。采用改良抗球蛋白试验，使用胶体金标记的抗人IgG，揭示血小板抗原与血小板反应性同种异体或自身抗体之间的抗原-抗体反应。将免疫金染色（IGS）与血小板悬液免疫荧光试验（PSIFT）以及固相红细胞粘附试验（SPRCA）进行比较。比较新鲜和冷冻保存血小板的反应性。
IGS的敏感性被证明比PSIFT和SPRCA略低（10%）：新鲜和冷冻保存细胞获得的结果评分相同。
冷冻保存血小板的免疫金染色检测似乎是血小板血清学的一种便捷技术方法。
