一种用于风疹病毒检测的蚀斑法。
A plaque method for rubella virus assay.
作者信息
ROZEE K R, GIVAN K F, KOANE F W, RHODES A J
出版信息
Can Med Assoc J. 1963 Aug 17;89(7):314-5.
A method has been described in which suitable dilutions of rubella virus will induce the formation, in a monolayer of green monkey kidney cells, of islets of infected cells which were protected from the effects of Echo 11 challenge virus. The number of islets or "negative" plaques was proportional to the dilution of rubella virus inoculated on to the monolayer. Using this method, it was observed that bentonite adsorption increased the plaque assay values of rubella virus pools. This suggested that rubella virus interference may be mediated by an interferon-like principle.
已描述了一种方法，在该方法中，风疹病毒的适当稀释液会在单层绿猴肾细胞中诱导形成感染细胞岛，这些细胞岛可免受埃可11型攻击病毒的影响。细胞岛或“阴性”噬斑的数量与接种到单层细胞上的风疹病毒稀释度成正比。使用该方法观察到，膨润土吸附增加了风疹病毒悬液的噬斑测定值。这表明风疹病毒干扰可能由一种类干扰素物质介导。
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