经胰蛋白酶水解后骨髓瘤球蛋白的本斯-琼斯蛋白与L多肽链的比较
Comparisons of Bence-Jones proteins and L polypeptide chains of myeloma globulins after hydrolysis with trypsin.
作者信息
SCHWARTZ J H, EDELMAN G M
出版信息
J Exp Med. 1963 Jul;118(1):41-53. doi: 10.1084/jem.118.1.41.
Abstract
L polypeptide chains of myeloma globulin and Bence-Jones protein isolated from the same patient were found to be identical after comparison of their tryptic hydrolysates by two-dimensional high voltage electrophoresis. The patterns of peptides from proteins belonging to antigenic group I differed markedly from those of proteins in antigenic group II. A partially purified H chain fraction was compared with L chains from the same myeloma protein. The tryptic hydrolysates yielded dissimilar patterns of peptides. These data indicate that gamma-myeloma proteins contain two kinds of polypeptide chains, Hgamma chains and either L(I) or L(II) chains. The L chains appear to be identical with those comprising the Bence-Jones protein from the same patient.
摘要
通过二维高压电泳比较同一患者分离出的骨髓瘤球蛋白和本-周蛋白的胰蛋白酶水解产物后发现，它们的L多肽链是相同的。来自抗原I组蛋白质的肽图谱与抗原II组蛋白质的肽图谱明显不同。将部分纯化的H链组分与来自同一骨髓瘤蛋白的L链进行比较。胰蛋白酶水解产物产生了不同的肽图谱。这些数据表明，γ-骨髓瘤蛋白含有两种多肽链，即Hγ链和L（I）链或L（II）链。L链似乎与来自同一患者的本-周蛋白中的L链相同。
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