Cianfriglia M, Viora M, Tombesi M, Merendino N, Esposito G, Samoggia P, Forsberg U H, Schröder J
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
Int J Cancer. 1992 Oct 21;52(4):585-7. doi: 10.1002/ijc.2910520416.
A panel of mouse x human B- and T-cell hybrids was analyzed for the expression of MC56 determinant which marks the drug-sensitive state of CEM cells. Karyotypic and phenotypic analyses of the tested clones showed that the expression of MC56 determinant correlated to the presence of human chromosome 11 and segregated concordantly to the epitopes recognized by monoclonal antibodies in the CD44 cluster. By using a particular class of interspecific rodent x human-cell hybrids in which the human genome counterpart is represented in the different clones only by human chromosome 11 or its fragments, we showed that the gene encoding for MC56 determinant is located on the region p13-pter of the short arm of chromosome 11. Therefore, the hypothesized homology between the drug-sensitivity marker MC56 and the CD44 determinant is supported also by gene mapping studies.
对一组小鼠与人类的B细胞和T细胞杂交体进行了分析，以检测MC56决定簇的表达，该决定簇标志着CEM细胞的药物敏感状态。对测试克隆进行的核型和表型分析表明，MC56决定簇的表达与人类11号染色体的存在相关，并且与CD44簇中单克隆抗体识别的表位一致分离。通过使用一类特殊的种间啮齿动物与人类细胞杂交体，其中人类基因组对应物在不同克隆中仅由人类11号染色体或其片段代表，我们表明编码MC56决定簇的基因位于11号染色体短臂的p13-pter区域。因此，基因定位研究也支持了药物敏感性标记MC56与CD44决定簇之间的假设同源性。
