Catmull J, Dan Z, Miller D J
Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.
Int J Parasitol. 1992 Aug;22(5):685-7. doi: 10.1016/0020-7519(92)90021-c.
mRNA from uterine microfilariae of the cattle parasite Onchocerca gibsoni was used for the construction of cDNA libraries. A cDNA clone encoding an antigen recognized by serum from human individuals infected with O. volvulus was found to contain five copies of an 87 bp unit. These 87 bp units were present in the genome in high copy number as long tandem arrays. These are the first cDNA sequence data obtained directly from larvae of any Onchocerca species.
来自牛寄生虫吉氏盘尾丝虫子宫微丝蚴的mRNA被用于构建cDNA文库。一个编码被感染旋盘尾丝虫的人类个体血清所识别的抗原的cDNA克隆被发现包含5个87 bp单位的拷贝。这些87 bp单位以长串联阵列的形式在基因组中高拷贝数存在。这些是直接从任何盘尾丝虫物种的幼虫获得的首批cDNA序列数据。
