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盘尾丝虫感染性幼虫抗原编码表达性cDNA克隆的分离与鉴定

Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.

作者信息

Unnasch T R, Gallin M Y, Soboslay P T, Erttmann K D, Greene B M

机构信息

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

出版信息

J Clin Invest. 1988 Jul;82(1):262-9. doi: 10.1172/JCI113581.

DOI:10.1172/JCI113581
PMID:2455736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC303503/
Abstract

The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult O. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated lambda RAL-1 was characterized further. The recombinant antigen produced by lambda RAL-1 stimulates proliferation of peripheral blood mononuclear cells from O. volvulus infected humans. Lambda RAL-1 is derived from a 1450 bases message that encodes a protein with an apparent molecular weight of 42,000 in adult O. volvulus. The inserted DNA of lambda RAL-1 contains an open reading frame of 1008 bp. The amino acid sequence predicted by this open reading frame contains three repeats of the sequence KKPEDWD. The identification of clones such as lambda RAL-1 will provide quantities of purified antigens sufficient to begin to study the immune response to and explore the development of immunity against the infectious form of the parasite.

摘要

本文描述了表达旋盘尾丝虫感染性幼虫中发现的抗原的重组cDNA克隆的分离。为了分离此类克隆，用针对感染性幼虫产生的抗血清筛选了一个由旋盘尾丝虫成虫RNA构建的表达cDNA文库。对一个命名为λRAL-1的克隆进行了进一步表征。λRAL-1产生的重组抗原可刺激来自旋盘尾丝虫感染人类的外周血单个核细胞增殖。λRAL-1来源于一条1450个碱基的信使RNA，该信使RNA在旋盘尾丝虫成虫中编码一种表观分子量为42,000的蛋白质。λRAL-1的插入DNA包含一个1008 bp的开放阅读框。该开放阅读框预测的氨基酸序列包含序列KKPEDWD的三个重复。鉴定如λRAL-1这样的克隆将提供足够数量的纯化抗原，从而开始研究针对该寄生虫感染形式的免疫反应并探索免疫的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/c8a4f5fa86e6/jcinvest00079-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/68a773498f04/jcinvest00079-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/483d6ca1eb59/jcinvest00079-0272-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/a8ba416b8b21/jcinvest00079-0272-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/40218e42bf4a/jcinvest00079-0272-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/d70b860c8b6c/jcinvest00079-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/f9d8ffbbd093/jcinvest00079-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/720632c7bb28/jcinvest00079-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/c8a4f5fa86e6/jcinvest00079-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/68a773498f04/jcinvest00079-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/483d6ca1eb59/jcinvest00079-0272-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/a8ba416b8b21/jcinvest00079-0272-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/40218e42bf4a/jcinvest00079-0272-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/d70b860c8b6c/jcinvest00079-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/f9d8ffbbd093/jcinvest00079-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/720632c7bb28/jcinvest00079-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935a/303503/c8a4f5fa86e6/jcinvest00079-0275-a.jpg

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